Four hundred strains isolated from Tunisian sheep and goat raw milks were initially screened for their ability to survive the GastroIntestinal Tract (GIT). Forty-three among the four hundred bacteria were resistant to pepsin, pH 2, pancreatin and bile salts at 0. 3%, even after 5 hours of incubation. Identification using 16S rRNA gene sequencing was established and we obtained as a species Lactobacillus plantarum (29 isolates from sheep milk and 11 from goat milk) and Lactobacillus pentosus (2 isolates from sheep milk and 1 from goat milk). We showed the ability for auto-aggregation and/or hydrophobicity properties. Finally, both M63 and C78 strains showed an important level of adhesion to three intestinal epithelial cells Caco-2 TC7, HT29-MTX, and HT29-CL. 16E. Taken together, these properties allow the lactobacilli strains to be considered promising beneficial strains for developing functional foods for consumers.

Background and Objectives: The aim of this study was to assess protection mechanisms against Escherichia coli by analyzing aggregation, adherence, antagonistic activity and safety characteristics of potentially probiotic Lactobacillus acidophilus B103. Materials and Methods: Potentially probiotic L. acidophilus B103 has been investigated in various aspects. Studied key characteristics included its pH and bile resistance, cell surface hydrophobicity and aggregation, cholesterol removal ability, hydroxyl radical scavenging activity and adhesion ability to Caco-2 cell monolayers. Antagonistic activity of the strain was assessed by adhesion competition on E. coli via competition, inhibition and replacement assays. Moreover, safety characteristics were investigated through DNase, hemolytic activity, biogenic amine production and antibiotic susceptibility assays. Results: L. acidophilus B103 included high stability to acidic pH (2.5 and 4.5), simulated gastric and intestinal juices and bile salt concentrations (up to 5% w/v). Moreover, L. acidophilus B103 showed relatively high hydrophobicity (51.79%), auto-aggregation (42.38%), co-aggregation (34.48%), cholesterol removal (46.27%) and hydroxyl radical scavenging activities (51.36%). Competition, replacement and inhibition anti-adhesion assays of the strain against E. coli were 52.13, 25.20 and 46.40%, respectively. Furthermore, L. acidophilus B103 adhered to simulated epithelial cells with a capacity of 11.85%. Neither DNase nor hemolytic activity was observed in the bacterial strain and the strain was highly sensitive to ciprofloxacin. Conclusions: Based on the results, safety assessments and its food origin, L. acidophilus B103 demonstrates potential for use in the food industry. It can serve as starter culture, co-culture and bio-protective agent, effectively enhancing safety and quality of the food products.

This paper introduces the concept of auto–Engel polygroups via the heart of hypergroups and investigates the relation between of auto–Engel polygroups and auto–nilpotent polygroups. Indeed, we show that the concept of heart of hypergroups plays an important role on construction of auto–Engel polygroups. This study considers the notation of characteristic set in hypergroups with respect to automorphism of hypergroups and shows that the heart of hypergroups is a characteristic set in hypergroups.

Morphology characteristic of rivers are always changing because of the dynamic nature of the rivers. These changes can effect and destroy agricultural areas and other land uses near the riverbanks. In this research, morphology of parts of Khoramabad River in Lorestan province from Ghaghakhor to Gholaman-e-Sofla (19.5 Km length) was studied. Using the aerial photos of the year 1955, TM Landsat images of the year 1988 and Aster images of the year 2005, three periods of river course were digitized in ArcGIS software. Then with the use of AutoCAD software, some geometrical parameters such as wavelength, valley length, sinuosity coefficient, central degree and radius of circle contact with arc were extracted for some analysis. Also for statistical analysis of the geometrical parameters of the river, we used SPSS software. The result of parameters study, mentioned above, and comparison of parameters in these ranges of time (1955-2005) showed that 17 meanders have been omitted from 1955 to 1988 and two meanders have been added from 1988 to 2005. Geometric parameters of three periods showed the changes on the river shape and pattern. The main reasons of these changes are land use on the riverbank and human impact on the riverbed and riverbank. The main reason of changes in the initial and middle of the study area are extension of Khoram Abad city and construction of buildings at the riverbank. The main reasons of changes at the end of the river, in river morphology are changes in land use and new tectonic movements. Also in all course of the river, human intervention is obvious in the changes of the river morphology.

Scene investigation is principal measure in forensic medicine. Study of body position in scene and situation of environment, collection of any stain spot, measuring of temperature, moisture, time, … can be helped to express about cause of death.Sixty years old women be died after self- burning, on the basis of police report. Observed forensic examiner was one room 3 4m, without any carpet and without soot or smut on the wall and roof. The body was carbonized at poligistic attitude with rupture of abdomen and visceral expose.Main task of forensic pathologist is determine of cause of death but expressing about manner of death too Important. Crime scene is not always place of body detection rather probability of crime appearing on the one place and transmission of body to anothers site.One the basis of information and after autopsy and judicial investigation, diagnosis of burning after murder of women is confirmed.

IntroductionCronobacter sakazakii is an opportunistic pathogen, which has been linked to the contamination of powdered infant formula, and associated with outbreaks leading to fatalities in neonatal intensive care units. Few studies have explored the direct interaction between probiotics and C. sakazakii. In this study, the effect of a Lactiplantibacillus plantarum strain (M17) along with the standard strain Lactobacillus plantarum (ATCC 8014) and the well-characterized probiotic strain Lactobacillus rhamnosus GG on the adhesion of C. sakazakii to intestinal epithelial cells was analyzed. Materials and MethodsAcid and bile tolerance of M17 was evaluated in the presence of pepsin and pancreatin. L-arginine hydrolysis was investigated using an arginine-including medium. Auto-aggregation and co-aggregation assays were performed by absorbance measurement. Minimum inhibitory concentrations of the antimicrobials recommended by the European Food Safety Authority were established. Total lactic acid and the ratio of D/L lactate isomers were determined with a Megazyme enzymatic kit. The ability of the isolate to produce biogenic amines was tested by qualitative and quantitative monitoring. Hemolysis was assessed phenotypically on MRS agar enriched with sheep blood. The strain was tested for its capability to adhere to mucin and Caco-2 cells. The antagonistic effects of the strain against C. sakazakii were further evaluated in vitro on mucin and cultured Caco-2 cells. The LAB strain was added simultaneously with, before, and after C. sakazakii to Caco-2 cells for competition, exclusion and displacement assays, respectively. Data analysis was performed in R using one-way analysis of variance, and the experimental groups were compared with the controls using Tukey’s test. P values <0.05 were considered statistically significant. Results and DiscussionThere was no significant difference in the survival rate of M17 and L. plantarum ATCC 8014 at pH = 4. After 2 h of incubation at pH = 2.5, the survival rate of L. plantarum ATCC 8014 was estimated to be higher than strain M17, but this difference was not significant. After 4 hours of incubation at pH = 8, M17 showed a higher survival rate than L. plantarum ATCC 8014, and this difference was significant after transfer from pH = 4. These results confirm the appropriate viability of M17 in the gastrointestinal tract. Both M17 and L. plantarum ATCC 8014 developed the color yellow in the L-arginine hydrolysis assay, which confirms the safety of these strains. The percentage of auto-aggregation for M17, L. plantarum ATCC 8014, and Lactobacillus rhamnosus LGG was estimated at 24.38, 25.28, and 32 after 6 hours, respectively, and no statistically significant difference between the two isolates were noticed. Given the auto-aggregation and co-aggregation parameters of M17, this strain may constitute a defense mechanism against C. sakazakii. Strain M17 showed resistance to kanamycin and clindamycin antibiotics. With intrinsic resistance, the risk of transferring resistance genes is not only speculative, but practically impossible. Intrinsic resistance of lactic acid bacteria may be considered desirable because it ensures their survival when the host is treated with antibiotics. Both D and L isomers of lactic acid were produced by the studied strains. In humans, D(-)-lactic acidosis is a rare metabolic complication that has only been reported in individuals with short bowel syndrome). Clinical studies have shown that the consumption of probiotic bacteria producing D(-)-lactic acid is safe for children and does not cause a long-term increase in blood D(-)-lactic acid. The reference L. plantarum strain and M17 did not produce biogenic amine precursors, and had no ß-hemolytic activity. Mucin adhesion assay exhibited that M17 has less adhesion (12.10 ± 1.14 %) than L. plantarum ATCC 8014 (13.33 ± 2.30 %) and LGG (15.93 ± 2.06 %) although these differences were not statistically significant. However, the amount of adhesion for the positive control sample Escherichia coli K12 (25.19 ± 4.40 %) was significantly higher than those of the other strains. Compared to the positive control, M17 had a significantly lower adhesion rate (6.8 ± 1.41) to CaCo-2 cells. This value was estimated at 13.77 ± 3.53 % for the reference strain and 21.6 ± 7.54 % for Lactobacillus fermentum PCC (positive control). In antagonistic assays, M17 was able to reduce the adhesion of C. sakazakii to mucin and CaCo-2 cells in all three methods of exclusion/inhibition, competition and displacement. Statistical analysis of the results does not show a significant difference between M17 and LGG. Therefore, the performance of M17 is similar to that of the standard probiotic LGG. ConclusionLactic acid bacteria with acceptable ability to adhere to epithelial cells can be suitable for colonization in the intestine. They can act as a barrier to fight pathogens through various competitive mechanisms, such as co-aggregation with pathogens and adhesion. The M17 strain has an acceptable immune profile and probiotic properties because it shows an acceptable antagonistic activity against C. sakazakii invasion. AcknowledgementThis study was supported by Ferdowsi University of Mashhad (Research affairs) [project No.:46718] and the research infrastructure at the University of Copenhagen.

Probiotics have been used for over a century to prevent and treat diseases. They can reduce the effects of gastroenteritis and are now used to treat acute diarrhea. This study aimed to evaluate the co-aggregative effects of probiotics bacteria against diarrheal causative bacteria. For this purpose, 11 isolates of probiotic bacteria were used in the current study, including three Lactobacillus plantarum, one Lactobacillus gasseri, two Lactobacillus fermentum, three Lactobacillus acidophilus, and two Lactococcus garvieae isolates. All isolates were tested for antibiotic susceptibility, autoaggregation ability, adhesion ability, antibacterial activity, acid tolerance, and bile salts tolerance. The results showed that most of them had the ability to autoaggregate after 4 h, with the highest percentage of 57.14% for L. fermentum. For the antibiotic susceptibility test, all the isolates showed resistance against trimethoprim/sulfamethoxazole, except one isolate. Moreover, all the isolates, except one, were susceptible to both vancomycin and tetracycline. All tested isolates had adhesion ability with different survival rates, which reached 34.57% for L. plantarum in acidic conditions. Besides, the highest survival rate was 85.17%, which belonged to L. garvieae, for bile salt tolerance. Probiotic isolates had an antibacterial effect against diarrhea-causative bacteria with an inhibition diameter of 17-49 mm for different Lactobacillus spp. and Lactococcus spp. isolates. Furthermore, the co-aggregation ability of probiotic isolates against diarrhea-causative bacteria was studied, and results showed that probiotic isolates had a co-aggregative effect against diarrhea-causative bacteria, Escherichia coli, Shigella sonnei, and Providencia alcalifaciens, after 24 h of incubation. The highest co-aggregative effect of probiotics isolates belonged to L. fermentum and L. acidophilus against P. alcalifaciens with a co-aggregation percentage of 100%, while the lowest co-aggregation rate was 14.29% against E. coli.  The findings revealed the probiotic properties and co-aggregative effects of probiotic bacteria against diarrhea-causative bacteria.